SiriusXT | Integrating Lab-Based Cryo Soft X-ray Tomography into CLEXM Workflows for 3D Correlative Imaging

Booth 1340

While electron microscopy offers exceptional subcellular detail, it is limited by demanding preparation, restricted field of view, and limited 3D context. Soft X-ray tomography (SXT) overcomes many of these challenges by imaging fully hydrated, cryo-preserved samples in 3D with minimal preparation and native contrast. The lab-scale SXT-100 microscope now enables integration into correlative workflows with light and electron microscopy. We present recent advances in CLEXM workflows, combining cryo-FM, SXT, and FIB/SEM or cryo-ET, highlighting their power for high-resolution, 3D imaging of cells and tissues in near-native state.